Suggestions for Positive Control Tissue Section

 

Suggestions for Control Section Material

 

Roy C. Ellis

IMVS Division of Pathology

The Queen Elizabeth Hospital

Woodville Road, Woodville, South Australia 5011

Email: roy.ellis@imvs.sa.gov.au

 

 

 

Acetylcholinesterase

Cerebellum

Acid phosphatase

Prostate, liver or kidney

Adenosine triphosphatase

Muscle or liver
Adrenaline Adrenal medulla
Alkaline phosphatase Kidney or small intestine
Alphafoetoprotein Primary carcinoma of liver
Aluminium Lung or skin in diseased patient
Amyloid AA

Widespread but kidney, liver, spleen, lymph nodes, adrenal and thyroid are classically involved.

Amyloid AL

Widespread but heart, gastro-intestinal tract, peripheral nerves, skin and tongue are classically involved.

Argentaffin cells

Appendix or ileum

Arginine

Paneth cells and lymphoid tissue

Argyrophil cells

Stomach, small intestine

Arsenic

Hair, nails and bone

Ascorbic acid

Liver or adrenal cortex

Aspergillus

Lung in diseased patient

Astrocytes, fibrous

Brain white matter

Astrocytes, protoplasmic

Brain grey matter

Axons

Cerebrum, cerebellum, spinal cord

Basement membranes

Kidney

Beryllium

Lung in diseased patient

Bilirubin

Biliary cirrhosis, cholelithiasis

Biliverdin

Biliary cirrhosis, cholelithiasis

Calcitonin

Dog thyroid

Calcium carbonate

Bone, necrosis, atheroma

Calcium oxalate

Kidney or thyroid in oxalosis, bone

Calcium phosphate

Bone, necrosis, atheroma

Candida albicans

Skin of diseased patient

Carcino-embryonic antigen

Colon

Carcinoid

Appendix, small intestine, rectum and sigmoid colon.

Cartilage, fibrous

Invertebral discs

Cartilage, hyaline

Joints

Charcot-leyden crystals

Lung of asthmatics, eosinophil granulomas

Chitin

Hydatid cysts of liver and lung

Cholesterol

Adrenal cortex and atheroma

Chromaffin

Adrenal medulla

Collagen

Skin, lung or liver
Copper

Liver in diseased patient - Wilson's disease, primary biliary cirrhosis

Cryptococcus

Lung of diseased patient

Cysteine

Hair follicles

Cystine

Stratum corneum and hair shafts

Cytochrome oxidase

Liver, muscle and kidney

Degenerate myelin

Brain of patients with multiple schlerosis

Dehydrogenase

Liver, kidney and heart

Dendrites

Cerebrum, cerebellum, spinal cord

DNA

Lymph node

Dopamine

Mast cells of mice and rats

Elastic fibres

Aorta, dermis of skin, lung or intestine

Epidermis, intercellular

bridges

Squamous cell papilloma, palmar and plantar skin

Esterase (non specific)

Liver, kidney or small intestine

Fatty acids

Fat necrosis

Fibrin

Foetal lung in hyaline membrane disease

Ganglion cells

Walls of the gastro-intestinal tract

Glucose-6-phosphatase

Colon

Glycogen

Liver

Golgi apparatus

Neurones

Gram negative organisms

Controls can be manufactured using a culture from a known organism and lung from autopsy. Culture together for 24 hours then fix and process.

Gram positive organisms

As for gram negative organisms

Haematoidin

Infarcts and abscesses

Haemazoin

Liver of malaria patients

Haemosiderin

Haemorrhage and haemochromotosis

Hepatitis B surface antigen

Liver in viral hepatitis

Human chorionic

Placenta gonadotrophin

Hyaluronic acid

Umbilical cord

Iron

Liver in hemochromatosis

Keratin

Palmar and plantar skin

Legionella pneumophilia

Lung of diseased patient

Leprosy bacilli (myco leprae)

Skin of diseased patient

Lipid

Corpus luteum of ovary

Lipofuschin

Heart, ganglion cells

Mallory bodies

Liver from alcoholic cirrhosis

Mast cells

Gastro-intestinal tract, intermuscular tissue or skin

Melanin

Negroid skin, naevus tumours

Microglia

Brain

Mitochondria

Renal tubules, liver and heart

Mucin

Small intestine, submaxillary gland

Mucopolysaccharides

Skin, umbilical cord

Muscle, striations

Skeletal muscle

Myelin

Cerebrum, cerebellum or spinal cord

Neurosecretory substance

Hypothalmus

Neutral fat

Subcutaneous tissue

Neutral mucin

Stomach

Nissl

Brain or spinal cord

Noradrenaline

Adrenal medulla

6-nucleotidase

Thyroid or liver

Osteoid

Bone in osteomalacia

Oxytalan fibres

Periodontal ligaments

Paneth cells

Small intestine

Peroxidase

Granulocytes, erythrocytes

Phospholipid

Brain or spinal cord

Plasma cells

Rheumatoid synovitis, lymph nodes in plasmacytosis

Pneumocystis carinii

Lung in diseased patient

Purkinje cells

Cerebellum

Purkinje fibres

Heart (subendocardium)

Reticulin fibres

Liver, spleen, lymphoid tissue

Ribonucleic acid

Plasma cell, neurones

Rickettsiae

Brain of diseased patient

Russell bodies

Rheumatoid synovitis

Seratonin

Small intestine (argentaffin cells)

Sialomucin (sialidase

labile)

Salivary gland

Sialomucin (sialidase

resistant)

Colon

Spirochaetes

Lyophilised T. Pallidum reconstituted, smeared on to clean glass slide and air dried.

Steroids

Adrenal cortex, seminiferous tubules

Sulphated mucin

Cartilage, colon

Tryptophan

Paneth cells, pancreas

Tubercle bacilli

Tissue from diseased patient, commonly lung

Tyrosinase

Skin (melanocytes)

Tyrosine

Pancreas

Urates

Gouty tophi

Viral inclusion body

Surface lesions of diseased patient (herpes). Lung of diseased patient (cytomegalovirus)

 

 

 

Reference

  1. Bancroft JD, Stevens HC. Manual of Histologic Techniques. Churchill Livingstone 1984

  2. Fischer RS, Petty CS. Forensic Pathology U.S. Department of Justice, Washington D.C. 1977

  3. Robbins SL, Angell M, Kumar V. Basic Pathology, W.B. Saunders. 1981

  4. Tedeschi CG, Eckert WG, Tedeschi LG. Forensic Medicine. W.B. Saunders. 1977

  5. Wheater PR, Burkitt Hg, Daniels VG. Functional Histology: A text and colour atlas. Churchill Livingstone. 1987

  6. Churukian CJ. Manual of the special stains laboratory. The Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York. 1993