White Labs Protocols

Adenovirus Protocols

• PLAQUE ASSAYS FOR ADENOVIRUS TITRATION

• Preparation of High Titer Adenovirus in P11 cells

Bacterial Protocols

• Bacterial Expression of GST-fusion Proteins

• Bacterial Expression of IRS-1 containing GST-fusion Proteins

• Denaturing purification of insoluble bacterial GST-fusion Proteins

• Preparation of LB and LB+ Amp Plates

DNA/RNA Isolation and Analysis

• Isolation of total RNA from mouse muscle

• PHENOL-CHLOROFORM EXTRACTION

• Large DNA Prep/CsCl

• Preparation of Herring Sperm DNA for Southern Blotting

• Probe DNA Preparation for Southern Blotting

• SDS Page

• Southern Blot Protocol

• Synthesis of Double-Stranded cDNA from Purified Poly(A) mRNA

• Oligos into Linkers

ERROR ANALYSIS

• Error Propagation

GENECHIP ANALYSIS

• ChipInfo

• dCHIP

• GO Surfer

GENOTYPING

• Multiple Genotyping Protocols

GENOTYPING MOUSE TAIL DNA

• SYSTEMIC IRS1 and IRS2

• SYSTEMIC JNK 123

• SYSTEMIC Insulin related receptor (IRR)

• SYSTEMIC PTP1B

• SYSTEMIC PDX1 KO and PDX1 transgene

• SYSTEMIC IGF1R

• SYSTEMIC IRS4

• SYSTEMIC IRS3

• RIP IRS2 Transgene

• FLOXED IRS2

Histology and Staining

• Immunocytochemistry for Paraffin-embedded Sections

Hormone Assays

• ELISA Protocol

Immunoprecipitation and Western Blotting

• ACTIVATION OF p70s6k and MAP KINASES

• GENERAL PROTOCOL FOR IMMUNO-

• IN VIVO PHOSPHORYLATION/IP

• SDSPAGE

• STAINGEL

• Tryptic digest of SDS-PAGE Purified proteins

• WESTERN BLOTTING PROTOCOL

• WESTBLOT 7/28/98

• FOXO1 WESTERN BLOT

• IMMUNOPRECIPITATION

Instrument Manuals

• Microbeta Workstation v4.0

• ImageQuant TL v2003.03

• Fragment User Guide

• ImageQuant Utilities

Kinase and Phosphatase Assays

• ACTIVATION OF p70s6k and MAP KINASES:

• Akt/PKB kinase assay

• In Vitro MAP kinase assay 9/1998

• IN-VITRO KINASE ASSAY

• JNK Kinase Assay

• JNK Kinase Assay

• PROTOCOLS 1

• PROTOCOLS 1

Live Animal Protocols

• Glucose tolerance test and fasting insulin test protocol (updated)

• GTT / ITT Data form

• Insulin tolerance test (updated)

• DEXA scanning protocol

Mammalian Cell Culture and Manipulation

• 35 S Met/Cys label Fao cells

• General Outline of Lipogenesis Assay (DRAFT VERSION)

• Anchorage-Independent Growth Assay

• Mammalian Cell Transfection – Calcium Phosphate Method

• Crystal Violet Staining (for Focus Formation Assay)

• General Protocol for an Epo Binding Assay with 293 Cell Line

• Glucose Transport Assay

• GROWING CHO CELLS

• FAOGROW

• RATGROW

• 32DGROW

• \PROTOCOLS\HIS100

• METHIONINE LABEL/IP

• MITOGENIC ASSAY FOR 32-D CELLS

• PC12 cell protocols

• Preparation of Neo (G418) Stock:

• Radiolabeled Ligand Binding Assay on 32D Cells

• PROTOCOLS\

• VEGF release assay

• WEHICM

Molecular Biology Protocols

• WHITE LAB – SOUTHERN BLOTTING PROTOCOL

• PCRMUT1/27/93

• LASER CAPTURE DISSECTION

OpenLab

• Open lab Preferences Setup

• Create a MAC Account for OpenLab

• Connecti OpenLab/MAC to WHITELABS

PCR Protocols

• Kyle’s protocol for long PCR

• PCR GENOTYPING PROTOCOL

• IRS-1 and IRS-2 Genotyping (PCR)

• PCRMUT1/27/93

• PCR Genotyping of Mouse Tails for IRS-1 and IRS-2

• RECOMBINANT PROTEIN FORM

Protein Protocols

• PROTOCOLS 1

• PROTOCOLS 1

• PROBING SH2 DOMAIN-CONTAINING

• PRODUCTION OF RECOMBINANT (BACULOVIRUS PRODUCED) IRS-1 PROTEIN

• ANTIBODY PURIFICATION

Silicon Genetics

• Manual Version 6.1

• Genet Version 4.1

Southern Blotting Protocols

• WHITE LAB – SOUTHERN BLOTTING PROTOCOL

• Preparation of Herring Sperm DNA for Southern Blotting

• Probe DNA Preparation for Southern Blotting

• Protocol for Genotyping by PCR

• SOUTHERN BLOTTING ON GENOMIC DNA

• SOUTHERN PROBE DNA PREPARATION

• WHITE LAB – SOUTHERN BLOTTING PROTOCOL

• WHITE LAB – SOUTHERN BLOTTING PROTOCOL

Typhoon

• Fluorescence Imaging With Typhoon

• ECL Plus Western Blot Detection

• Detection of Biomolecules Part 1

• Detection of Biomolecules Part 2