Vodkin Lab Protocols

 

Dr. Lila Vodkin

Department of Crop Sciences

College of Agricultural, Consumer, and Environmental Sciences

University of Illinois at Urbana-Champaign

 

 

• Preparation of DNA for microarrays

• PCR Data/Reaction Sheet

• Electrophoresis of PCR products

• Purification of PCR products with Sephadex

• Transferring PCR DNA from 96 to 384 well plates

• Preparing poly-L-lysine coated slides

• Postprint slide treatments

• Postprint blocking of amine slides

• Postprint blocking of aldehyde slides

• Reverse transcriptase labeling with Cy3/Cy5

• Staining of total DNA on slides

• Microarray construction

• Laser scanning

• Spot quantitation

• Statistics of expression

• Sample tracking