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Segall Lab Protocols
Anca M. Segall
Professor of Biology
University of Utah, Center for Microbial Sciences
Segall Lab Protocols
Large scale Int purification (3-100 liters)
PDF
Small scale Int purification (0.25-3 liters)
PDF
IHF purification protocol
PDF
Xis purification protocol
PDF
Western protocol
PDF
Int recombination and bandshift buffers
PDF
Making electrocompetent cells
PDF
Buffers
PDF
PFGE
PDF
MIC
PDF
Microscopy
PDF
TUNEL DNA Damage Detection Assay (updated)
DOC
96 Well Template (updated)
XLS
Patching Grid (updated)
PPT
Small scale electroporation
PDF
Viability Experiment
PDF
Antibiotic Information (updated)
DOC
6X Loading Dye for DNA
DOC
Alkaline lysis plasmid miniprep
DOC
B-Galactosidase assay - Microtiter plate format
DOC
Genomic DNA preparation (CTAB method)
DOC
Chemically-Competent Cell Recipe
DOC
EBU plates
DOC
Electrocompetent cells
DOC
Protocol for removing markers flanked by FRT sites
DOC
NCE Medium
DOC
P1 Transduction (E. coli)
DOC
DNase-free RNase
DOC
Sam's Red Swap Guide
DOC
Arbitrary PCR
DOC
4X SDS-PAGE Sample Loading Buffer
DOC
Pfx50 Polymerase Formula
XLS
Taq Formula
XLS