Schedl Lab Protocols

• Ballistic transformation and Supplement

• Dissection, staining, in situ

• Gonad dissections
• Gonad fixation
• DAPI staining
• Antibody staining of dissected gonads
• Antibody staining for activated MPK-1 (MAPK-YT, Diphospho-MPK-1)
• Germline in situ hybridization
• Worm powder
• RNAi stuff
• RNAi plates with lactose (instead of IPTG)
• Feeding RNAi
• dsRNA (double-stranded RNA) for injection
• Genomic stuff
• Worm genomic DNA prep
• Single worm PCR
• Mutagenesis stuff
• Mutagenesis with EMS
• Mutagenesis with ENU
• Transformation stuff
• Yeast transformation
• Electrocompetent cells
• Heat shock transformation (DH10B homemade)
• Protein stuff
• BSA conjugation with maleimide (Pierce)
• Glycine elution (affinity purification)
• IPTG induction
• Kinase assay
• Cytosolic extract & immunoprecipitation
• Cytosolic extract using sonication (fast)