Rossant Lab Protocols

Whole Mount in Situs & Immunohistochemistry

• DPERK whole-mountimmunohistochemistry

• Combined DPERK whole-mount immunohistochemistry and in situ hybridization

• double in situ hybridization (post-implantation)

• double fluorescent in situ hybridization (peri-implantation)

• whole-mount antibody staining

• whole-mount BrdU staining

• microarrays

• Axiocam MR camera instructions

Plasmid Maps and Sequences

• map and sequence information :pHspLacZpA (plasmid #213)

• hsp 68 sequence information

• siRNA information

• siRNA map

Protocols (PDF)

• whole-mount in situ hybridization / Conlon version

• combined in situ procedures / Conlon version

• whole-mount in situ hybridization / Henrique version

• whole-mount in situ hybridization / deRobertis version

• clearing with BABB

• dissections