Home
IHC Store
Products
Services
Knowledge
Protocols
Forum
Gallery
Jobs
Distributors
Advertise
Blog
Contact
Rosen Lab Protocols
Jeffrey M. Rosen, Ph.D.
C.C. Bell Professor of Molecular and Cellular Biology
Baylor College of Medicine, Houston, Texas
Rosen Lab Protocols
Adenovirus infection of primary MECs
Avertin
(anesthetic for mice)
ChIP Assay
(Chromatin immunoprecipitation assay)
Freeze mammary gland pieces
(for later transplantation)
Hematoxylin & Eosin
(staining of tissue sections)
Hormone treatment, acute
(2 day E+P) plus
BrdU injection
Immunostaining
tutorial
Immunostaining:
dilutions and conditions
Immunostaining:
Representative images
Immunofluorescence:
General protocol
Immunohistochemistry:
General protocol
I
mmunohistochemistry:
"Milk Method"
Immunoprecipitation Assay
(for Western blotting)
Masson Trichrome staining
(stain for collagen)
Paraformaldehyde, 4%
(tissue fixative)
Primary MEC isolation
(mammary epithelial cells)
Primary MEC extracts
(for Western blots/IP assays)
Progesterone receptor/BrdU double immunofluoresence
Retroviral infection of tissue culture cells
TUNEL assay
(
in situ
detection of apoptotic cells)
Wholemount staining
(hematoxylin staining of whole mammary glands)
Wholemount staining II
(carmine alum staining of mammary glands)
X-Gal staining of tissue
(to detect LacZ expression)
Bacterial beta-Galactosidase Histochemisty Bible