Thomas Ried, M.D.
Section of Cancer Genomics
Genetics Branch, Center for Cancer Research, NCI, NIH, DHHS
PDF Isolation of Interphase Nuclei from Paraffin Section for
FISH, Hedley Technique
PDF Metaphase Preparation from Mouse Embryonic Fibroblast (MEF)
PDF Preparation of Paraffin Sections and Frozen Tissue for FISH
PDF Prometaphase Chromosome Preparation from Mouse Spleen
(C57Bl/6)
FISH -
Fluorescence In Situ Hybridization
CGH -
Comparative Genomic Hybridization
MicroArrays -
Expression & CGH
Immunocytochemistry/Molecular Biology
PDF Western Blot Analysis: Detection of Proteins using Alkaline
Phosphatase
PDF Western Blot Analysis: Detection of Proteins using
Chemiluminescence