• PDF High Resolution Chromosomes

• PDF Isolation of Interphase Nuclei from Paraffin Section for FISH, Hedley Technique

• PDF Metaphase Preparation from Adherent Cells

• PDF Metaphase Preparation from Mouse Embryonic Fibroblast (MEF)

• PDF Metaphase Preparation from Mouse/Rat Spleen

• PDF Preparation of Human Metaphase Chromosomes

• PDF Preparation of Paraffin Sections and Frozen Tissue for FISH

• PDF Prometaphase Chromosome Preparation from Mouse Spleen (C57Bl/6)

• PDF Storage of Chromosome Preparations

• PDF Triple Enzyme Mouse Tumor Digestion

DNA Probe Preparation

• PDF cDNA Synthesis for RT-PCR Protocol

• PDF DNA Preparation from Adherent Cells

• PDF DNA Preparation from Blood

• PDF DNA Preparation from Cell Lines, High Salt Method

• PDF DNA Preparation from Fresh/Frozen Tissue

• PDF DNA Preparation from Paraffin Tissue

• PDF Isolation of BAC DNA using Autogen 850

• PDF Preparation of Alpha-satellite DNA by PCR

• PDF Primary Amplification of Genomic DNA using DOP-PCR

FISH - Fluorescence In Situ Hybridization

• PDF Antifade

• PDF DNA Precipitation and Hybridization (FISH)

• PDF FISH Detection

• PDF Nick Translation (FISH)

• PDF SKY/FISH of Previously G-banded Slides

• PDF Slide Pretreatment

• PDF Tyramide Signal Amplification (TSA) Detection

CGH - Comparative Genomic Hybridization

• PDF Antifade

• PDF CGH Detection

• PDF DNA Precipitation and Hybridization (CGH)

• PDF Nick Translation (CGH)

• PDF Slide Pretreatment

SKY - Spectral Karyotyping

• PDF Amplification of Flow Sort DNA using DOP-PRC (SKY)

• PDF Antifade

• PDF DOP-PCR Labeling (SKY)

• PDF DOP-PCR Secondary (SKY)

• PDF Hybridization (SKY)

• PDF Precipitation of SKY Kits and QC

• PDF Primary Amplification Genomic DNA using DOP-PCR

• PDF SKY Detection

• PDF SKY/FISH of Previously G-banded Slides

• PDF Slide Pretreatment

MicroArrays - Expression & CGH

• PDF cDNA Microarray Indirect Labelling

• PDF RNA Extraction from Cell Lines or Frozen Tissue

Immunocytochemistry/Molecular Biology

• PDF Immunocytochemistry

• PDF Immunocytochemistry Followed by Electron Microscopy

• PDF Immunocytochemistry Followed by FISH (Version 1)

• PDF Immunocytochemistry Followed by FISH (Version 2)

• PDF Immunocytochemistry Followed by FISH (Version 3)

• PDF Preparation of Protein Extracts for 2D Gel-analysis

• PDF RT_PCR Reaction

• PDF Western Blot Analysis: Detection of Proteins using Alkaline Phosphatase

• PDF Western Blot Analysis: Detection of Proteins using Chemiluminescence

• PDF Western Blot Analysis: Preparation of Protein Extracts

• PDF Western Blot Analysis: Protein Quantification

Miscellaneous

• PDF Collagen Coating Flasks

• PDF Giemsa-trypsin Banding