Dr. Craig S. Pikaard
Biology Department, Washington University in St. Louis
Broccoli (Brassica oleracea) RNA polymerase I holoenzyme purification (PDF file)
Promoter-independent RNA polymerase activity assay (PDF file)
Promoter-dependent in vitro transcription and S1 nuclease protection assay (PDF file)
Transient expression in Arabidopsis protoplasts (PEG method)
Chromatin immunoprecipitation and ChIP-chop (PDF file)
Bisulfite-mediated DNA sequencing for mapping methylated cytosine positions (PDF file)
Pulsed-field gel electrophoresis of Arabidopsis chromosomal DNA (PDF file)
Purification of core histones from Broccoli (Brssica oleracea) (PDF File)
Olga Pontes' protein immunolocalization and DNA-FISH methods (PDF file)
Isolation of genomic DNA and total RNA from same total nucleic acid prep
Single leaf DNA prep for Southern blot analysis of transgenic plants in first generation
Affinity purification of anti-peptide antibodies (PDF file)