Pikaard Lab Protocols

 

Dr. Craig S. Pikaard

Biology Department, Washington University in St. Louis

 

 

 

Pikaard Lab Protocols

 

Gene Expression Methods

Chromatin Methods

  • Chromatin immunoprecipitation and ChIP-chop (PDF file)

  • Bisulfite-mediated DNA sequencing for mapping methylated cytosine positions (PDF file)

  • Pulsed-field gel electrophoresis of Arabidopsis chromosomal DNA (PDF file)

  • Purification of core histones from Broccoli (Brssica oleracea) (PDF File)

Cytogenetics and Cell Biology Methods

Nucleic Acid Purification

Antibody methods

  • Affinity purification of anti-peptide antibodies (PDF file)

Agrobacterium tumefaciens methods