Olson Lab Protocols

 

 

 

 

Olson Lab Protocols

 

Immunocytochemistry

• Hemotoxylin and Eosin Staining

• Ki-67 Staining

• Ubiquitin Aggregate Staining

• NeuN BrdU Double Staining

• Immunostaining Cells (Notch 18G)

• Neuronal Markers - Great Summary

RNA/DNA/cDNA

• Genomic Southern Blot

• RNA Prep/cDNA Synthesis for RT-PCR

Tissue Culture/Primary Cultures

• Luciferase Assay

• Primary Tumor Preparation/NCAM/ATP Assay

Proteins

• Comprehensive Protocol: SDS-Polyacrylamide Gel & Western Blot (from JS)

• Western Blot (from JP)

• Protein Preparation from Cells