NIEHS Lab Protocols

 

National Institute of Environmental Health Sciences

 

Laser Microdissection Protocols

• Discussion of Laser Microdissection Projects
• Identifying Areas To Microdissect
• Optimal Tissue Collection for Laser Microdissection
• Laser Microdissection Cryostat Section Preparation
• Frozen Tissue Storage
• Laser Microdissection Staining of Frozen Sections
• Paraffin Block Preparation for Laser Microdissection
• Laser Microdissection Paraffin Slide Preparation
• Laser Microdissection Staining of Paraffin Sections
• Reagents & Supplies

Flow Cytometry Protocols

• Annexin V Protocols
• Calcium Flux Analysis
• CaspaTag Caspase Activity Protocol
• DNA Analysis by Flow Cytometry
• FASC Analysis of Cell Cycle using BrdU and PI
• Immunofluorescent Staining of Rat Thymocytes
• Membrane Potential Analysis by Flow Cytometry
• PARP PE
• Sodium and Potassium Analysis by Flow Cytometry

Immunohistochemistry Protocols

• Manual Tissue Staining Protocols
• Automatic Tissue Staining Protocols
• Manual Cell Staining Protocols

Microarray Protocols