Electrocompetent
E.coli cells
Bacterial
electroporation
Preparation of
media
Preparation of agar plates
SDS-PAGE on vertical mini gels
Preparation of affinity
resins
Protein purification using the
NiNTA resin
Preparation of phage particles from phage vectors
Running
2D-PAGE
Silver staining of 2D-gels
Coomassie staining of 2D-gels
Scanning 2D gels
In-gel digestion of proteins
SYPRO Ruby Protein Gel Stain
Affinity measurements by
band-shift assay
Affinity measurements by
competition ELISA
Protein expression in
Pichia
Culturing HEK 293 Cells
Transfection of kidney cell line.
Generating
stable cell lines in HEK293
Protocols for the preparation of
tumour cells for s.c. injections
Preparation of
cryopreserving medium
Protocols for the preparation of cryotubes of
tumour cells
Protocols for the
CAM assay
Protocols for the
immunohistochemistry
Pellet preparation for the cornea micropocket
assay
DNA sequencing using the ABI Prism sequencer
ETH-2 library protocols: on-line or in Word format