Dr. Dyche Mullins, UCSF
Protein Purification
antibody purification
GST fusion expression
NWASp purification using urea
WWA purification and coupling
mini arp2/3 prep protocol
capping protein purification
alpha-actinin purification
poly-l-proline columns
purification of profilin
Lab Stocks
competent cells
preparation of dialysis tubing
making ATP stocks
making buffer A stocks
making TCEP stocks
Assays
pyrene fluorescence I
pyrene fluorescence II
General
accession numbers
extinction coefficients
Other
SDS PAGE protocol
activated CH4B sepharose
cyanogen bromide cleavage
fixing & immunofluorescence
fixing & permeabilization for IF
HPLC nucleotides
immunoprecipitations
making an antibody
membrane floatation
DNA, RNA & SS oligo quantification
SOEing PCR
thrombin cleavage
fransformation of bacteria
typical cloning strategies
yeast transformation
