Prokaryotic Cells
Phage Preparation and Purification
Preparation of Competent Cells
Transformation
Eukaryotic Cells
Cell Culture
Cell Transfection
Establishing Stable Cell Lines
Radiolabeling
Cell Growth/Death Assays
Luciferase Assays
Cell Imaging
Protein Biochemistry
Protein Expression and Purification
Protein Analysis
Electrophoresis
Antibodies
DNA-Protein Interactions
Protein-Protein Interactions
Re-Folding Assays
DNA Techniques
Plasmid Preparation
Mini prep
Large prep
Preparation of DNA from Cultured Cells
DNA Electrophoresis
Cloning Techniques
Fragment isolation
Sequencing
Modification
Amplification
RNA Techniques
RNA Isolation
RNA Electrophoresis
Polysome Analysis of RNA
Transcriptional Analysis
Nucleic Acid Hybridization
Yeast Methods:
The
Forsburg lab website is an excellent source of S.
pombe information:
Yast-Two-Hybrid Interaction Trap: See the protocols developed by
the
Brent
lab
C. elegans Methods
General
Extraction
Staining
Analyses
Genetics
RNAi
Appendix
4.
Photography
5.
Densitometer