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Molecular Biology Lab Manual
Julie B. Wolf
Department of Biological Sciences, UMBC
CHAPTER 1:
General Laboratory Methods
Safety Procedures
Preparation of Solutions
Disposal of Buffers and Chemicals
Equipment
-
Micropipets
-
Using a pH meter
-
Autoclave operating procedures
-
Operating instructions for spectrophotometer
Working with DNA
Sterile Technique
CHAPTER 2:
Instructions for Notebook Keeping
CHAPTER 3:
Computer User's Guide
CHAPTER 4:
Molecular Biology Methods
M.1:
Preparation of genomic DNA from bacteria
M.3:
Restriction enzyme digestion of DNA
M.4:
Phenol/chloroform extraction of DNA
M.5:
Ethanol
precipitation of DNA
M.6:
Agarose
gel electrophoresis
M.7:
Transformation of E. coli by
electroporation
M.8:
Wizard PCR
preps DNA purification system
M.9:
Alternate method for purifying DNA from
agarose gels
M.10:
Transfection of mammalian cells using
Lipofectamine (LTI)
M.11:
Southern
blotting
M.12:
RT-PCR Protocol
M.13:
Preparation of
sequencing gels
M.14:
Isolation of RNA from
mammalian cells using RNAZOL (Teltest)
CHAPTER 5:
Tissue
Culture Methods
Types of cells grown in culture