Laurie Lab Protocols

• Preparation of Broth etc.

• Mini-Plasmid Preparation

• Plasmidprep for Sequencing

• Preparation of Competent Cells

• Transformation Protocol

• Salmon-sperm DNA Preparation

• c-DNA Library Screening

• Excision of Insert from lambda to pBluescript

• Nested-deletion Protocol

• Manual Sequencing Protocol

• Northern Hybridization

• Southern Hybridization

Cell Biology Protocols

• Preparation of BMS Extract

• Cell Adhesion Protocol

• Lacrimal Experimental Protocol

• Thawing and Freezing Cells

• Identification / Purification of SN-Peptide Receptor

• Coupling of SN-Peptide -Sulpholink

• Immunostaining of Cells Adherent to Coverslips

• Protein Gels / Electrotransfer

• Silver Staining

• Immunostaining of Paraffin Sections

• BCA Protein Determination Method

• ELISA