Gordon Laurie, PhD
Associate Professor of Cell Biology
University of Virginia
Laurie Lab Protocols
Preparation of Broth etc.
Mini-Plasmid Preparation
Plasmidprep for Sequencing
Preparation of Competent Cells
Transformation Protocol
Salmon-sperm DNA Preparation
c-DNA Library Screening
Excision of Insert from lambda to pBluescript
Nested-deletion Protocol
Manual Sequencing Protocol
Northern Hybridization
Cell Biology Protocols
Preparation of BMS Extract
Cell Adhesion Protocol
Lacrimal Experimental Protocol
Thawing and Freezing Cells
Identification / Purification of SN-Peptide Receptor
Coupling of SN-Peptide -Sulpholink
Immunostaining of Cells Adherent to Coverslips
Protein Gels / Electrotransfer
Silver Staining
Immunostaining of Paraffin Sections
BCA Protein Determination Method