For details on working on with transiently transfected and stably expressed GFP-tagged proteins (including sections on live cell imaging, FRAP and FRET by acceptor photobleaching and FLIM/FRET), download our recent Methods in Molecular Biology chapter here.
Fixation, permeabilization and immunostaining of cells (contains images and movies)
Nucleolar isolation (contains images and movies)
Cajal body isolation (contains images and movies)
For details on cell fractionation and preparation of whole cell vs. nuclear and cytoplasmic lysates, download our Cell Fractionation Protocol.