Kearney Lab Protocols

 

Dr. John F. Kearney, Professor

Department of Microbiology, University of Alabama at Birmingham

 

 

 

Kearney Lab Protocols

• Recipes

Flow Cytometry

• Compensation with CellQuest

• Intracellular Staining for FACS

• Using CellQuest

ELISA

• Dex ELISA

• Spot ELISA

Microscopy

• Cytospin Protocol

• Cytospin Protocol for Tdt

• Immunofluorescence Staining

Hybridomas

• Hybridoma Clones Available

• Screening for PC abs

• Subcloning of Hybridomas

Others

• Antibody Purification

• Spore Preparation

• Embedding Spleen

• Subtractive Immunization

• Fusion of Fetal/Neonatal Livers or Spleens

• Tissue Culture

• Intranasal Innoculation of Mice

• LPS Activation

• R36a Preparation