Select one of the protocols below to download a
pdf version.
EQUIPMENT
How to build an
inebriometer
PDF (908 KB)
ASSAYS
Inebriometer use
PDF (60 KB)
Inebriometer cleaning
PDF (60 KB)
Booze-o-mat use: Tracking protocol
PDF (84 KB)
Booze-o-mat use: Video settings
PDF (88 KB)
Ethanol absorption assay
PDF (544 KB)
Protein assay
PDF (68 KB)
Lifespan experiment
PDF (68 KB)
Negative geotaxis "Bang" assay
PDF (96 KB)
Dopamine & 3IY feeding and locomotor assays
PDF (76 KB)
HISTOLOGY
Adult CNS dissection
protocol
PDF (1.7 MB)
Larval CNS dissection protocol
PDF (188 KB)
Antibody staining protocol
PDF (48 KB)
Dissection & antibody staining of
3rd instar larval bodywall or NMJ
PDF (568 KB)
Beta-galactosidase activity staining in CNS
PDF (52 KB)
Frozen head sections
PDF (252 KB)
MUTAGENESIS
Ethyl methanesulfonate (EMS) mutagenesis PDF (116 KB)
Hydroxyurea ablation of mushroom bodies PDF (84 KB)
PREPS
Fly food recipe PDF (76 KB)