Gottschling Lab Protocols

 

Dr. Gottschling, Fred Hutchinson Cancer Research Center

 

 

 

Gottschling Lab Protocols

 

Yeast Protocols

• Rather Rapid Genomic Prep

• Plasmid Rescue

• Protein Prep

• Yeast Immunoprecipitation

• Yeast Histone Prep (from Jakob Waterborg; .pdf format)

• Media Recipes

  • HC media

  • HC Dropout Plate Supplementation Table

  • YC media

  • YC Dropout Plate Supplementation Table

  • MET15 Resource center (lead plates)

• Sporulation and Examples of a Dissection

• Transformation

• Gene Disruption via PCR

• RNA Prep

• Quantitative Mating Assay

• Colony PCR

• Beta-gal Assay

• Yeast FACS Analysis

• Colony morphology

• Lab Lore

• Methods from the Fangman/Brewer Lab

• Methods from the Gietz Lab

Bacterial Protocols

• Mini-Preps

• Transformation

• M9 Plate Supplementation Table

General Protocols

• Big Dye Sequencing

• Southerns

• Northerns

• Non-radioactive probes

• Radioactive probes

• Protein protocols (Westerns, etc.)

• S1 Nuclease protection

• Inverse PCR

• Oatmeal Chocolate Chip Cookies

• In-gel hybridization for detecting telomeric single-stranded TG tails (Wellinger Lab)

• Download FileMaker Pro template for bacteria strains.

• Download FileMaker Pro template for yeast strains.

• Download Excel template for tetrad dissection results.