Goldberg Lab Protocols

 

 

 

Goldberg Lab Protocols

  1. Methods and Procedures

    1. Standard (alkaline lysis) mini-prep

    2. GeneClean© II kit

    3. Preparation of electrocompetent cells

    4. Electroporation

    5. ELISA inhibition assay

    6. Immunodot assay

    7. Transformation of competent cells (RbCl2 method)

    8. Partial endonuclease digestion

    9. Wizard© miniprep kit

    10. SDS-PAGE

    11. LPS recovery

    12. Lyophilizing cells

    13. Preparation of phage lysates

    14. Silver staining of PAGE gels

    15. Western blotting (semi-dry)

    16. Tricine SDS-PAGE

    17. Rapid genomic DNA isolation from P. aeruginosa

    18. Altered Sites© mutagenesis protocol

  2. Media, Buffer and Reagent Recipies

    1. Mini-prep solution I

    2. Mini-prep solution II

    3. Mini-prep solution III

    4. 3 M sodium acetate

    5. 10x TE buffer

    6. LB broth

    7. M9 media

    8. SOC broth

    9. 2 M magnesium stock

    10. 1 M glucose

    11. 300 mM sucrose

    12. p-nitrophenyl phosphate disodium substrate buffer

    13. Phospate buffer

    14. Skim milk solutions

    15. 10x PBS

    16. SOB broth

    17. Transformation buffer 1

    18. Transformation buffer 2

    19. 0.5 M MOPS

    20. 1 M potassium acetate

    21. 7.5 M ammonium acetate, pH 7.5

    22. 2 M ammonium acetate, pH 4.6

    23. SDS-PAGE separating gels

    24. SDS-PAGE stacking gel

    25. 30% acrylamide/0.8% bisacrylamide

    26. 4x Tris-Cl/SDS, pH 6.8

    27. 4x Tris-Cl/SDS, pH 8.8

    28. 5x SDS/electrophoresis buffer

    29. 2x PAGE sample buffer

    30. Terrific broth

    31. TNE buffer

    32. lambda-Medium

    33. lambda-Top agar

    34. lambda-Diluent

    35. 1 M magnesium sulfate

    36. Silver stain fixative I

    37. Silver stain fixative II

    38. Silver stain stop solution

    39. Silver stain kit reagents

    40. Western blot transfer buffer

    41. TSDS-PAGE separating gel monomer

    42. TSDS-PAGE stacking gel monomer

    43. TSDS-PAGE anode buffer

    44. TSDS-PAGE cathode buffer

    45. TSDS-PAGE gel buffer

    46. TSDS-PAGE stacking gel

    47. TSDS-PAGE separating gel

  3. Figures and Tables

    1. ELISA inhibition plate

    2. Partial endonuclease digestion diagram

    3. Separating gel table

    4. Calculating phage titers

    5. Phage media diagram

    6. Silver stain protocol

    7. Western blot transfer stack assembly

    8. Western blot transfer times

    9. DNA digestion using rapid P. aeruginosa genomic prep