Giorgi Lab Protocols

 

Dr. Janis V. Giorgi

Flow Cytometry Laboratory, UCLA

 

 

• Flow Cytometry Glossary
• Method for EtOH fixation of cells for long-term storage and DNA staining
• Minimizing Background Staining
• Blocking FC-Receptor Binding
• Monoclonal Antibody Staining Procedure
• Staining Procedure for Whole Blood
• Preparation of 2% Formaldehyde Solution
• Propidium Iodide Staining of Dead Cells
• 7-AAD Staining of Dead Cells
• DNA Staining for Cell Cycle Analysis
• Intracellular Antigen Staining
• Surface Antigen Staining and DNA Content
• Questions and Answers about Cell Sorting
• Surface Staining for Cell Sorting
• Simultaneous Measurement of Apoptosis and Necrosis
• Detection of Apoptosis by 7-AAD Staining
• CD38 Fluorescence Intensity Quantitation
• Calibrating Becton Dickinson Flow Cytometers for RFI Measurements
• Measurement of GFP Expression and DNA Content in Permeabilized Cells
• Measurement of Green Fluorescent Protein Expression and DNA Content in Unfixed Cells
• Simultaneous Measurement of Cell Surface Immunofluorescence, Viability, and DNA content
• Simultaneous Measurement of Dual-Color Immunofluorescence and DNA/RNA Content
• Simultaneous Measurement of Three-Color Immunofluorescence and DNA Content
• Measurement of Intracellular CA2+ and Cell Surface Antigen Expression