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Flemington Lab Protocols
Dr. Flemington, Tulane Health Sciences Center
DNA Methods
Generation of vector based siRNAs
Plasmid Subcloning
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General Cloning Tips
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Plasmid Subcloning Troubleshooting Guide
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Plasmid subcloning using "Low Melt" ligation protocol
Bacterial Transformation Protocols and Generation
of Competent Cells.
Plasmid Preps
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Plasmid Micro Prep
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Plasmid Mini Prep (Alkaline lysis).pdf
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Plasmid Mini Prep (Boil Method).pdf
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CsCl2 Maxi Plasmid DNA Prep.pdf
Genomic DNA Preps
Southern Blot Analysis
DNA Labeling Methods
DNA Sequencing
RNA methods
Gene Regulation Methods
Analysis of Endogenous Cellular Gene Expression
- S1 Nuclease Analysis of mRNA levels.pdf
- Data Base of S1 Nuclease Oligos.pdf
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Western Blot Analysis of Endogenous Gene Expression.pdf
Footprinting Assays
In Vitro Transcription Protocol
Transient Reporter Analysis
Antibody Methods
Cell Manipulation methods
Transfection Protocols
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Calcium Phosphate Transfection Method.pdf
- Electroporation
- Superfection
Retroviral Transduction Methods
Table of Drug Sensitivities for Stable Cell Line
Selection
Cell cycle Analysis
Maintenance Conditions for Special Cell Lines
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Maintenance of IMECs (immortalized mammary epithelial cells).pdf
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Culture Conditions for various stable cell lines.pdf
EBV Methods
Virus Preparation