Flemington Lab Protocols

 

Dr. Flemington, Tulane Health Sciences Center

 

 

DNA Methods

Generation of vector based siRNAs

Plasmid Subcloning

Bacterial Transformation Protocols and Generation of Competent Cells.

Plasmid Preps

Genomic DNA Preps

Southern Blot Analysis

DNA Labeling Methods

DNA Sequencing

RNA methods

Gene Regulation Methods

Analysis of Endogenous Cellular Gene Expression

Footprinting Assays

In Vitro Transcription Protocol

Transient Reporter Analysis

Antibody Methods

Cell Manipulation methods

Transfection Protocols

Retroviral Transduction Methods

Table of Drug Sensitivities for Stable Cell Line Selection

Cell cycle Analysis

Maintenance Conditions for Special Cell Lines

EBV Methods

Virus Preparation