Fangman/Brewer Lab Protocols

DNA Preps

• Yeast "smash-&-grab" DNA prep

• Yeast genomic DNA prep for 2-D gels

• Preparation of yeast DNA embedded in agarose plugs

• Gel-purified, agarased YACs for yeast transformation

2-D Gel Electrophoresis

• Standard 2-D gels

• Fork-direction gels

Replication Timing

• Density transfer

• Comparative hybridization

• Transient hemimethylation

Transformation

• Yeast transformation using frozen competent cells

• Gel-purified, agarased YACs for yeast transformation