Dr. Maitreya Dunham, Lewis-Sigler Fellow
Lewis-Sigler Institute for Integrative Genomics
Carl Icahn Laboratory, Princeton University
Almost all the microarray protocols have been updated, either to accommodate Agilent's latest randomization of their reagents or to circumvent recurring artifacts (the CGH protocol in particular). Please note that I didn't cut these protocols from whole cloth. They are mostly derived from the work of others, plus my own amendments and annotations.
Protocols are specifically for yeast, but they also work for other species. Also, the Agilent protocols are for 4x44k arrays, but it turns out all that needs adjusted for the different platforms is the volume, not the amount of labeled material. Check the Agilent manuals for recommended volumes.
Ozone and Cy5 [HTML]