Acetate NaCl buffer ( .htm)

Acrylamide ( .htm)

Affinity purification of antibodies ( .htm)

Alkaline carbonate buffer ( .htm)

Ammonium acetate ( .htm)

Blunt ending vectors ( .htm)

Blunting a restriction digest ( .htm)

Blunting a PCR fragment ( .htm)

Blue/white screening ( .htm)

Calcium chloride ( .htm)

cDNA Library Screening (Filter washings) ( .htm)

CHO cells

        cell maintenance ( .htm)

        transfection ( .htm)

Cloning blunt ends ( .htm)

CNBr Sepharose Handbook (Amershamm-Pharmacia, *.pdf)

Continuous semidry transfer ( .htm)

Coomassie stain ( .htm)

Cos-7 cells 

           IFAs ( .htm)

          cell maintenance ( .htm)

          RNA prep ( .htm)

Counting trypanosomes ( .htm)

Coupling Antigen to CNBr sepharose ( .htm)

Cytomix ( .htm)

Cytoskeleton

        immunofluorescence assay ( .htm)

        Cytoskeleton Prep (trypanosomes) ( .htm)

DMSO stocks of Trypanosomes ( .htm)

DNA

        loading buffer 10X ( .htm)

        maxi prep ( .htm)

        miniprep ( .htm)

vaccination of mice ( .htm)

Gutat10.1 cDNA library ( .htm)

Drug cocktail for triple selection ( .htm)

E. Coli transformation ( .htm)

EDTA (0.5 M) ( .htm)

Ethidium Bromide staining trypanosomes ( .htm)

Fetal calf serum ( .htm)

Freeze-squeeze DNA isolation ( .htm)

Frozen stocks of trypanosomes

        DMSO ( .htm)

        Glycerol ( .htm)

Gel Mobility Shift Assay ( .htm)

Genomic DNA from Tryps ( .htm)

Gluteraldehyde (0.5%) ( .htm)

Glycine

        100 mM ( .htm)

        for quenching PFA ( .htm)

         pH8 in Coupling buffer ( .htm)

Grunstein Transfers ( .htm)

Heat inactivating fetal calf serum ( .htm)

HEPES.KOH (5 mM) ( .htm)

HMI-9 media for BSF T. brucei

Hypotonic lysis buffer (J. Bangs) ( .htm)

Imidizole dialyzing buffer ( .htm)

Immunofluorescence Assay

        Immunofluorescence assay ( .htm)

        solutions ( .htm)

        on isolated flagella ( .htm)

on isolated cytoskeletons ( .htm)

Lab Saftey Instructions

Laemmli buffers for SDS_PAGE ( .htm)

LB media ( .htm)

LDNT media for T. cruzi culturing

Lower SDS_PAGE Gels (Resolving) ( .htm)

Lysis buffer (5X) (J.Bangs) ( .htm)

Magnesium Acetate ( .htm)

Magnesium Chloride ( .htm)

Manganese Chloride ( .htm)

MSMS Saftey Data Sheets

Microscope Sign Up Sheet (Excel worksheet)

MME (cytoskeleton buffer) ( .htm)

Mouse Bleeds

    retro-orbital mouse bleed ( .htm)

    terminal mouse bleed ( .htm)

Nickle Column buffers ( .htm)

Northern blot protocol ( .htm)

NZY Top Agar ( .htm)

Paraformaldehyde (2%) ( .htm)

PBS (phosphate buffered saline) ( .htm)

PCR colony lysis buffer ( .htm)

PEME ( .htm)

PMN (cytoskeleton) ( .htm)

Poly-acrylamide ( .htm)

Polymerase chain reaction  ( .htm)

Poly-l-lysine cover slips ( .htm)

Potassium chloride  ( .htm)

Potassium phosphate stock ( .htm)

Procyclic trypanosome methods ( .htm)

Protein Purification Handbook (Amersham-Pharmacia, *.pdf)

PS (phosphate buffered sucrose) ( .htm)

Qiagen buffer P1 ( .htm)

Qiagen buffer P2 ( .htm)

Qiagen buffer P3  ( .htm)

Qiaquick gel purification  ( .htm)

Rapid silver stain for SDS-PAGE ( .htm)

Recombinant Protein Handbook (Amersham-Pharmacia, *.pdf)

Restriction endonuclease digestion ( .htm)

RNA prep from Cos-7 cells ( .htm)

RPMI media for mammalian cell culture

SDM-79 (PCF T. brucei medium) ( .htm)

SDS-PAGE

      buffers (Laemmli) ( .htm)

      gels for Western ( .htm)

Semi-dry protein transfer ( .htm)

SeqWeb Analysis                                                    

Shrimp Alkaline Phosphate buffer ( .htm)

SM medium (Cunningham’s) ( .htm)

Sodium acetate ( .htm)

Sodium bicarbonate buffer ( .htm)

Sodium chloride ( .htm)

Sodium Phosphate (0.1M) ( .htm)

Southern Blot protocol ( .htm)

T4 blunting a restriction digest ( .htm)

T4 blunting a PCR fragment ( .htm)

TAE (50X) ( .htm)

TBE (5X) ( .htm)

TBS (10X) ( .htm)

T. brucei

        Cytoskeleton Prep ( .htm)

        Drugs for stable trypanosome selection ( .htm)

        DMSO stocks ( .htm)

        Electroporation media ( .htm)

        Glycerol stocks ( .htm)

        Isolating genomic DNA from ( .htm)

        Procyclic trypanosome methods ( .htm)

        SM medium (Cunningham’s) ( .htm)

        transient transfection ( .htm)

T. cruzi

        Culturing ( .htm)

        Transfection (amastigotes) ( .htm)

TCA precipitation of protein ( .htm)

TE ( .htm)

Terminal mouse bleed ( .htm)

Tissue Culture plate conversion / specs ( .htm)

TRIS ( .htm)

Triton phosphate buffer for protein ( .htm)

Triton X-100 ( .htm)

Trypanosome transient transfection ( .htm)

Trypanosome frozen stocks (see Frozen stocks of trypanosomes)

Upper SDS-PAGE gel (stacking gel) ( .htm)

Western Blot

        Basic protocol ( .htm)

        wet transfer buffer ( .htm)

YEPD ( .htm)