Peter N. Devreotes, Ph.D.
Johns Hopkins University, School of Medicine
Devreotes Lab Protocols
Ammonium Sulfate Binding
GTPgS Activation of ACA
CHIFF Preparation
In vivo Actin Polymerization
Purification of cAR1 from CHIFFs
In vitro Actin Polymerization
MolBioProtocols
Fluorimeter Start and Shut Down
Western Blot of cAR1
FTPSWAP
Photo affinity Labeling of cAR1
Spot Screening of Phenotypes
Preparation of Cells with Upper Band and Lower Band
Electroporation
GTP Inhibition of Binding
Phagocytosis
Antibody Staining for Gold EM
Needle Assay for Chemotaxis
Bead Binding Assay
Screen of Colonies
Equilibrium Dialysis Binding
Random Mutagenesis
In vivo Activation of ACA
REMI
Crac Supernatant Preparation
In Vivo Translocation of CRAC