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De Robertis
Lab Protocols
Dr. Eddy M. De Robertis
Howard Hughes Medical Institute
De Robertis Lab Protocols
Fertilization of frog eggs
Primers for RT-PCR
Frog whole mount in situ hybridization
Mouse whole mount in situ hybridization
Paraplast in situ on sections
Cryostat in situ on sections
Skeletal staining
Alcian blue cartilage staining
RT-PCR
Animal Cap Assay
Dissociation and reaggregation of animal cap cells
Keller sandwich explant
Spemann's organizer transplantation
BDA lineage tracing on sections
Whole-mount lacZ lineage tracing
Hematoxilin and eosin staining
Whole-mount staining with Alkaline phosphotase antibodies
Natural and artificial fertilization
Western blot for phosphoprotein samples
Northern blot
In situ hybridizations on sections
In situ hybridization to detect miRNA expression in
Xenopus
using Locked Nucleic Acid probes
Xenopus
whole-mount immunostaining
Immunohistochemistry on Mouse Sections