De Robertis Lab Protocols

 

Dr. Eddy M. De Robertis

Howard Hughes Medical Institute

 

 

 

De Robertis Lab Protocols

• Fertilization of frog eggs
• Primers for RT-PCR
• Frog whole mount in situ hybridization
• Mouse whole mount in situ hybridization
• Paraplast in situ on sections
• Cryostat in situ on sections
• Skeletal staining
• Alcian blue cartilage staining
• RT-PCR
• Animal Cap Assay
• Dissociation and reaggregation of animal cap cells
• Keller sandwich explant
• Spemann's organizer transplantation
• BDA lineage tracing on sections
• Whole-mount lacZ lineage tracing
• Hematoxilin and eosin staining
• Whole-mount staining with Alkaline phosphotase antibodies
• Natural and artificial fertilization
• Western blot for phosphoprotein samples
• Northern blot
• In situ hybridizations on sections
• In situ hybridization to detect miRNA expression in Xenopus using Locked Nucleic Acid probes
• Xenopus whole-mount immunostaining
• Immunohistochemistry on Mouse Sections