Cossarizza Lab Protocols

 

Apoptosis: A Laboratory Manual of Experimental Methods

By Andrea Cossarizza & Diana Boraschi, Eds.

 

 

 

THE CELL

 

1. Morphological aspects of apoptosis Walter Malorni, Stefano Fais & Carla Fiorentini
2. Cell cycle Miriam Capri & Daniela Barbieri

 

THE NUCLEUS


3. Fluorescence microscopy analysis of nuclear alterations during apoptosis Ildo Nicoletti & Roberta Mannucci
4. Qualitative analysis of DNA fragmentation by agarose gel electrophoresis Paola Bossu’
5. Fluorochrome labelling of DNA strand breaks by terminal deoxynucleotidyl transferase (TdT-assay) and analysis by flow cytometry Ildo Nicoletti & Roberta Mannucci
6. A whole blood method for apoptosis determination Claudio Ortolani, Gabriella Scuccato, Roberto Cibin, Alessandro De Toffoli, Paola Arslan
7. Multiparametric analysis by flow cytometry Sandra Nuti
8. Detection of apoptosis in situ with immunocytochemistry and TUNEL Daniela Barbieri & Enzo Ottaviani
9. Quantitation of DNA fragmentation with diphenylamine Diana Boraschi & Giovanni Maurizi
10. APOPTOSIS vs. NECROSIS Marco Vitale, Giorgio Zauli & Elisabetta Falcieri

 

THE CYTOPLASM


11. Caspases Ann Zeuner, Maria Rita Rippo, Ruggero De Maria & Roberto Testi
12. Sphingomyelinases Luisa Di Marzio & Maria Grazia Cifone
13. Tissue transglutaminase Maria Elisabetta Cosulich & Marina Fabbi
14. Analysis of mitochondrial membrane potentialAndrea Cossarizza & Stefano Salvioli
15. Cytoskeleton Carla Fiorentini, Paola Matarrese, Alessia Fabbri & Walter Malorni

 

THE PLASMA MEMBRANE


16. ANNEXIN V Stefania Morrone
17. Differential light scattering and CD45 membrane fluorescence Maurizio Carbonari
18. Modification of antigen expression in apoptotic cells: a single laser methodology Filippo Reno’ & Stefano Papa