Chin-Sang Lab Protocols

• GFP SCOPE How to equip a Stereoscope for GFP fluorescence  for  under $200 (Using a super bright blue LED).

• Our Online Subscription to Molecular Cloning Manual

• 4D video microscopy

• 2X Rapid Ligation Buffer (just scale up to 5X if needed) NEB technical manual  Promega manual 

• 5X Long PCR Buffer

• Antibody Purification using blotted Antigen

• Antibody Staining mixed stage and embryos (2 days)   Embryo staining modified by S. Brisbin (up to 4 days)

• C. elegans total genomic DNA preparation

• Chemiluminescent Detection Recipe aka ECL

• Colloidal Coomassie Blue Stain for SDS PAGE gels

• Cosmid prep

• DiI DiO staining of amphid  neurons and phasmids (Michael Koelle)

• EMS mutagenesis protocol

• Frozen Yeast Competent Cells

• How to microinject worms   [Microinjection movie]

• Immunoprecipitation  protocol

• Lac Z liquid Assays (Y-PER method)

• Lethal phase determination (Michael Koelle's Lab)

• M9 plates -Leu for  E. coli

• Our Silica based plasmid prep

• PCR Fusion approach for reporter constructs (Hobert Lab)

• Purification of 6X His Proteins

• Recovering Yeast Two Hybrid plasmids from yeast  (non glass bead method)

• RNAi Plates

• Single Worm PCR 

• Super (frozen) Competent E. coli Cells

• T-A cloning vectors

• TMP/UV integration 

• RNA isolation (Trizol method)

• X-Gal Yeast Overlay Assay

• Yeast Transformation protocol   Yeast transformation solutions from The Gietz Lab

• C. elegans Protocols online

• PROTOCOLS FOR RECOMBINANT DNA ISOLATION, CLONING, and SEQUENCING
  by Bruce A. Roe, Judy S. Crabtree and Akbar S. Khan

• Chin-Sang lab  manual
  Microscopy 

• 4D video microscopy

• Adjusting the stereomicroscope for parafocality

• Installing an HBO mercury bulb (real video)

• Aligning an HBO mercury bulb (real video)

• practice Focus and alignment of mercury lamps

• "homemade" GFP scope

• How to microinject worms