GFP SCOPE
How to equip a Stereoscope for GFP
fluorescence for under $200 (Using a super bright
blue LED).
2X Rapid Ligation Buffer
(just scale up to 5X if needed)
NEB technical manual
Promega manual
Antibody Staining mixed stage and embryos
(2 days)
Embryo staining modified by S. Brisbin (up to 4 days)
DiI DiO staining of amphid neurons and phasmids (Michael
Koelle)
Lethal phase determination
(Michael Koelle's Lab)
Recovering Yeast Two Hybrid plasmids from yeast (non glass
bead method)
Yeast Transformation protocol
Yeast transformation solutions from The
Gietz Lab
PROTOCOLS FOR RECOMBINANT DNA ISOLATION, CLONING, and SEQUENCING
by Bruce A. Roe, Judy S. Crabtree and Akbar S. Khan
Chin-Sang lab manual
Microscopy
practice
Focus and alignment of mercury lamps