Professor Walter J. Chazin
Vanderbilt University, Center for Structural Biology
Chazin Lab Protocols
Standard Cell Prep
Preparation of Competent Cells (old)
Getting DNA sequenced
Isolating Inclusion Bodies
MALDI
Amino Acid Analysis
Minimal Media Recipe
Sterile Technique
Thrombin Cleavage
Western Blotting and Immunostaining
Limited proteolysis
Thermal melt using CD
Calculating denaturant concentration by r.i.
Theoretical simulations and considerations for Kd measurement and calculation
Protocol for growing labeled protein
RPA-AB Purification
T-Antigen Purification
DNA primase Exp. / Purification
Calbindin D9k Prep
updated Calbindin D9k Prep
Calmodulin Prep
S100A2 Prep
S100A6 Prep
S100B Prep
S100A8/A9 Prep
Production of Calbindomodulin Mutants
Centrin Prep
Purification Protocol for HCentrin2
CDPK prep
Competitive Chelator Method for Calcium-Binding Constants (Updated version)
Phenyl Sepharose Binding Assay for Calbindin Mutants
Preparing Chelexed water