Chazin Lab Protocols

General Procedures

• Standard Cell Prep

• Preparation of Competent Cells (old)

• Getting DNA sequenced

• Isolating Inclusion Bodies

• MALDI

• Amino Acid Analysis

• Minimal Media Recipe

• Sterile Technique

• Thrombin Cleavage

• Western Blotting and Immunostaining

• Limited proteolysis

• Thermal melt using CD

• Calculating denaturant concentration by r.i.

• Theoretical simulations and considerations for Kd measurement and calculation

• Protocol for growing labeled protein

DNA Processing Assemblies

• RPA-AB Purification

• T-Antigen Purification

• DNA primase Exp. / Purification

Calcium-Binding Proteins

• Calbindin D_9k Prep

• updated Calbindin D_9k Prep

• Calmodulin Prep

• S100A2 Prep

• S100A6 Prep

• S100B Prep

• S100A8/A9 Prep

• Production of Calbindomodulin Mutants

• Centrin Prep

• Purification Protocol for HCentrin2

• CDPK prep

• Competitive Chelator Method for Calcium-Binding Constants (Updated version)

• Phenyl Sepharose Binding Assay for Calbindin Mutants

• Preparing Chelexed water

• Walter's notes about NMR with EDTA