Immunohistochemistry Frequently Asked Questions (FAQ)

 

Immunohistochemistry

Frequently Asked Questions (FAQ)

 

Dr. Giorgio Cattoretti
Associate Professor of Clinical Pathology
Institute for Cancer Genetics, Columbia University
New York, USA

 

 


IHC FAQ Home

How stable are the diluted antibodies?

Answer:

 

Antibodies diluted as directed are very stable. Months and probably years. One studyRef has sown extended shelf life for commercial reagents, beyond the manufacturer's expiration date. For antibodies used very diluted against low level antigens (<2K molecules/cell) is probably safer make fresh solutions monthly.