Immunohistochemistry Frequently Asked Questions (FAQ)

 

Immunohistochemistry

Frequently Asked Questions (FAQ)

 

Dr. Giorgio Cattoretti
Associate Professor of Clinical Pathology
Institute for Cancer Genetics, Columbia University
New York, USA

 

 


IHC FAQ Home

Do I need to freeze my antibody aliquots?

Answer:

 

Each freezing cycle causes aggregation of the immunoglobulin and loss of titer. Avoid freezing aliquots that you expect to use out in the next couple of years. You may freeze reference reagents in small aliquots for reference or reagents to be stored without preservative.